Rini, Triani (2021) Synthesis of Protein Hydrolysate from the Prepupae of Hermetia illucens using a Papain Enzymes. Research Journal of Applied Sciences, Engineering and Technology, 18 (1). pp. 12-19. ISSN 2040-7467
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Abstract
Abstract The aim of this study was to determine optimum enzyme concentration and hydrolysis time needed to hydrolyze black soldier fly prepupae into a protein hydrolysate. The prepupae were homogenized with aquadest and papain enzyme (0-9% on a weight basis) at pH 7 for 24-72 h at 55°C. At optimum conditions (enzyme concentration of 6% on a weight basis and hydrolysis time of 24 h), the yield of protein hydrolysate was 31% on a dry weight basis. The protein content increased from 39.7 to 61% (dry weight basis) whereas the lipid content in the protein hydrolysate decreased from 30.3 to 0.5% (dry weight basis). Hydrolysis of the prepupae with a papain enzyme increased the amino acid content from 15.4 to 47.1% on a weight basis. The black soldier fly prepupae contains 15 detectable amino acids primarily consists of glutamic acid (10.7%), alanine (10.7%), leucine (10%) and valine (9%). After hydrolysis with a papain enzyme, the composition of the amino acid changed and primarily consists of glutamic acid (17.3%), tyrosine (9.4%), leucine (9.4%) and alanine (8.9%). The protein hydrolysate may find application as a supplement for feed and food for a better absorption of nutrients in animal intestine. Keywords: Amino acid, black soldier fly prepupae, enzymatic hydrolysis, papain, protein hydrolysate
| Item Type: | Article |
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| Subjects: | JOURNAL |
| Divisions: | Fakultas Teknik > Teknik Pangan 2021 |
| Depositing User: | Irwan Kustiawan Kosasih |
| Date Deposited: | 19 Jan 2022 05:22 |
| Last Modified: | 19 Jan 2022 05:22 |
| URI: | http://repository.unpas.ac.id/id/eprint/55119 |
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